COMPREHENSIVE ANALYSIS OF CHEMOKINES AND CYTOKINES SECRETED IN THE PERITONEAL CAVITY DURING LAPAROTOMY

Rei Kawashima, Yuki I. Kawamura, Tomoyuki Oshio, Noriko Mizutani, Toshihiko Okada, Yutaka J. Kawamura, Fumio Konishi, Taeko Dohi
2012 Journal of immunoassay & immunochemistry  
& We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before closure (postoperative), and were assayed for the presence of 27 cytokines and chemokines using multiplex beads assay. As a result, IL-6 and CCL5 showed the most striking increase during operation.
more » ... ombinant CCL5 or lavage fluid induced chemotaxis of human peripheral blood mononuclear cells. We propose that CCL5 is possibly involved in the mechanism of postoperative adhesion in humans.
doi:10.1080/15321819.2011.638409 pmid:22738652 pmcid:PMC3431549 fatcat:lplma4rruvaovbnmkmnjiyfibq