Ultrastructure Analysis of Primary and Permanent teeth with Generalized Amelogenesis imperfecta; In-vitro study

Ghada Hamed, Walid El-Sayed
2020 Egyptian dental journal  
Amelogenesis or enamel formation is a biomineralization process in which specialized epithelial derived cells, ameloblasts, secrete extracellular matrix which later will be mineralized. amelogenesis can be divided into two main stages: the secretory stage and the maturation stage. Objectives: This work aims to investigate the ultrastructure phenotype of permanent and deciduous teeth with generalized enamel defects. Material and Methods; Teeth whether deciduous or permanent (exfoliated or
more » ... xfoliated or extracted for clinical reasons), were collected from affected individuals. Scanning Electron Microscopy Energy Dispersive X-Ray Spectroscopy techniques were applied. Enzyme digestions was done by using either α-chymotrypsin (Sigma-UK) or lipase (Sigma-UK). Results: Examination of sections of affected teeth using Scanning Electron Microscopy showed abnormal enamel rods that were covered by amorphous material. This pattern was consistent of both primary and secondary dentitions. Energy Dispersive X-Ray Spectroscopy showed increase carbon contents. α-chymotrypsin enzyme was the only enzyme capable for removing this amorphous material. Conclusion: Ultrastructure analysis of the affected teeth showed typical form of hypomaturation amelogenins imperfecta. The study also proved that this amorphous material is mainly proteinases in nature.
doi:10.21608/edj.2020.24006.1016 fatcat:d5jfyhkbl5f37niolybanewxzm