Effect of Lupinus Growth Factor on the in vitro Growth of Embryos of Various Plants and Carrot Root Tissue

1964 Shokubutsugaku Zasshi  
Since coconut milk was shown to stimulate the growth of Datura embryo in vitro1 , the use of it has become common in the embryo and the tissue culture of various plants2-$~ . Similar growth-promoting factors were found in a number of plant materials9 -12, . In the present study the application of embryo factor obtained from young Lupinus seeds12> to the embryo culture of various plants other than Datura and to callus tissue of carrot root was investigated, and the distribution of embryo factors
more » ... n of embryo factors in various plant materials was examined. Materials and Methods Young embryos of Datura tatula, Pharbitis nil, Bidens biternata, Antirrhinum majus, Lupinus luteus, Stellaria media, Brassica campestris, Capsella bursa-pastoris, Astragalus sinicus, Vicia faba, Iris pseudoacorus, Vicia saliva, Triticum aestivum, and young hybrid embryos from a genetically incompatible cross of Brassica pekinensis (2n=40) x B. chinensis (2n=20), and callus tissue from the root of Daucus carrta were cultured. For the aseptic isolation of embryos, fruit of Datura was treated with 30 per cent ethanol for four minutes. The fruits of other plants and carrot root were treated additionally also with 10 per cent calcium hypochlorite solution for 20 minutes. Embryos were isolated as described by Van Overbeek et al.' Discs of carrot root of 4 mm diameter and 2 mm thickness were prepared with a cork borer and a knife. For embryo culture, the basal medium contained the modified white's solutionl2" 40 g sucrose, and 9 g agar per liter, unless otherwise stated. The medium was sterilized by autoclaving at 1.0 kg/cm2 overpressure for 10 minutes. For the tissue culture, the basal medium, of which sucrose concentration was 20 g/l, contained additionally 2 mg IAA, 3 mg glycine, 0.5 mg nicotinic acid, 0.1 mg thiamine-HC1, and 0.1 mg pyridoxine-HC1, and the medium was steam-sterilized at 100° for one hour. The seed diffusates of Lupinus luteus, Glyeine sofa, Phaseolus vulgaris var. humilis and "Black Valentine " , Pisum sativum, Arachis hypogaea, Canavalia ensiformis, and fruit dill usate of Brassica campestris were prepared as reported in the previous paperl2> . The Lupinus diff usate was autoclaved with the basal medium, unless otherwise stated. The dill usates of other plants were sterilized with Seitz-filter and added aseptically to the autoclaved basal medium shortly before solidification. Young embryos of Datura were cultured at 30° in darkness for 5 days, and those of other plants at 26° under continuous illumination of fluorescent lamps. The growth
doi:10.15281/jplantres1887.77.403 fatcat:elceihdi7bbgzbhg3zkqeklonq