Cytokine-based research is revolutionizing the treatment of several diseases including cancer, inflammation, infectious diseases, obesity, haematological disorders and other diseases affecting the immune system. The discovery and identification of new antitumor drug with low side effects on immune system has become an essential goal in many studies of immuno pharmacology. Present investigation was carried out to study the anticancer effects of Different nano compound against cell lines 4T1,

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... and L929.cell lines were used as biological models. Two lines were derived from breast cancer (4T1) and acute myeloid leukaemia (HL60). Another cell line was normal mouse fibroblast cell line (L929). Drugs was prepared in four concentrations (drug cncentration: 0.001, 0.01, 0.1,1 µmol/ml). The difference between groups was determined by analysis of variance (ANOVA). Cell lines incubated with different concentrations of drugs for 24 hours and cell growth inhibition was determined using MTT assay. Inhibitory effect of α-IFN-SWNT conjugated and encapsulated α-IFN in PLGA on three cell line, had shown significant differences (p<0.05). The maximum growth inhibitory effect was on HL60 cell line. All drugs induced a significant growth inhibition and apoptosis in a dose-dependent (drug cncentration: 0.001<0.01<0.1<1 µmol/ml). The results of this study indicate that nanoparticles could be a potentially useful delivery system for IFN as an anticancer agent and other anticancer drugs in treatment of leukemia.