Optimization of CTAB-based RNA Extraction Method for Aspergillus flavus

Virali Antala, Hemangini Chaudhari, Nidhi Radadiya, Hiral Desai, B. A. Golakiya, H. P. Gajera
2020 International Journal of Current Microbiology and Applied Sciences  
Journal homepage: http://www.ijcmas.com High-quality RNA extraction are very important for further downstream molecular applications such as transcript amplifications by reverse transcriptase-polymerase chain reaction (RT-PCR) and elaboration of cDNA for expressed gene study. Aspergillus flavus is the paramount aflatoxin producing fungus in cultivated oil seeds. The methodology with some modification is described here that allows the intact RNA isolation from mycelium. RNA quality was evaluated
more » ... by electrophoresis in agarose gel, quantitative Real-Time PCR of cDNAs derived from isolated mRNA and subsequent PCR amplification using primers designed against β-tubulin and aflO/ omtB/ dmtA/ O methyltransferase B from A. flavus. K e y w o r d s
doi:10.20546/ijcmas.2020.908.038 fatcat:wvd2gkandnd6xfwkfx3elkivim