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The aim of the present study was to compare two protocols of Agar Gel Immunodiffusion (AGID) that can be used to diagnose Equine Infectious Anemia (EIA). Different volumes of reagents and support for agar gel were used, as recommended by the Ministry of Agriculture, Livestock and Food Supply (MAPA) and the World Organization for Animal Health (OIE). Equidae sera samples (n = 1000) were tested using a national commercial kit and different volumes (i.e. 25 µL-MAPA and 50 µL-OIE) of reagents anddoi:10.4236/ojvm.2015.57023 fatcat:nmt7dq73ivcwpjauwnowsxoini