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Genetic diversity and molecular survey of Trypanosoma (Megatrypanum ) theileri in cattle in Brazil's western Amazon region

Thábata dos Anjos Pacheco, Arlei Marcili, Andrea Pereira da Costa, Rute Witter, Andréia Lima Tomé Melo, Ricardo Vilas Boas, Cristiane Silva Chitarra, Valéria Dutra, Luciano Nakazato, Richard de Campos Pacheco
2018 Revista Brasileira de Parasitologia Veterinária  

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Trypanosoma (Megatrypanum) theileri is a flagellated protozoan that infects ruminants and it displays high genetic diversity. In this study, we investigated the prevalence rates of this protozoan based on hemoculture and molecular diagnosis. The isolates of T. theileri thus obtained were characterized by molecular markers SSU rDNA and gGAPDH and molecular diagnosis based on Cathepsin L-like gene (PCR-TthCATL). The PCR-TthCATL and hemoculture indicated an overall prevalence rate of 8.13%, and
more » ... CATL derived sequence named IB was identified for the first time in cattle in the western Amazon region, as well as IF in Brazil. We also describe a possible new PCR-TthCATL derived sequence in cattle, designated IL.
doi:10.1590/s1984-296120180049 pmid:30133593 fatcat:s4s4oqqoojg2pjswrrs3cueuc4
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Thábata dos Anjos Pacheco, Arlei Marcili, Andrea Pereira da Costa, Rute Witter, Andréia Lima Tomé Melo, Ricardo Vilas Boas, Cristiane Silva Chitarra, Valéria Dutra, Luciano Nakazato, Richard de Campos Pacheco. "Genetic diversity and molecular survey of Trypanosoma (Megatrypanum ) theileri in cattle in Brazil's western Amazon region." Revista Brasileira de Parasitologia Veterinária 27.4 (2018)