Coconut oil cake-A novel substrate of solid state fermentation for the production of α α α α-amylase using Streptomyces spp

N Maheswari, S Soundariya
Journal of Chemical and Pharmaceutical Research   unpublished
In this present study Streptomyces was isolated from the Mangroves soil and identified by gram staining, biochemical tests and microscope observation. The optimum growth condition for the isolated organisms for the α-amylase production were such as pH, temperature, substrate concentration. On the production α-amylase using the isolated organisms the four different substrate such as under varying condition of coconut oil cake, groundnut oil cake, wheat bran, rice bran,were assessed coconut oil
more » ... ke was produced the maximum amount of α-amylase in, pH4(18.5±0.02) temperature25 0 C(8.5±0.02)substrate concentration 15g (12.5±0.02).Thus from the above study, it is concluded that coconut oil cake produces higher amount of α-amylase compared to groundnut oil cake, wheat bran, ricebran. Thin layer chromatographic the end products librated by to action of α-amylase to separate the glucose and maltose by using thin layer chromatographic.