In this present study Streptomyces was isolated from the Mangroves soil and identified by gram staining, biochemical tests and microscope observation. The optimum growth condition for the isolated organisms for the α-amylase production were such as pH, temperature, substrate concentration. On the production α-amylase using the isolated organisms the four different substrate such as under varying condition of coconut oil cake, groundnut oil cake, wheat bran, rice bran,were assessed coconut oil

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... ke was produced the maximum amount of α-amylase in, pH4(18.5±0.02) temperature25 0 C(8.5±0.02)substrate concentration 15g (12.5±0.02).Thus from the above study, it is concluded that coconut oil cake produces higher amount of α-amylase compared to groundnut oil cake, wheat bran, ricebran. Thin layer chromatographic the end products librated by to action of α-amylase to separate the glucose and maltose by using thin layer chromatographic.