Evaluation of single-nucleotide polymorphisms in CAPN1 for association with meat tenderness in cattle1,2

B. T. Page, E. Casas, M. P. Heaton, N. G. Cullen, D. L. Hyndman, C. A. Morris, A. M. Crawford, T. L. Wheeler, M. Koohmaraie, J. W. Keele, T. P. L. Smith
2002 Journal of Animal Science  
Micromolar calcium activated neutral protease (CAPN1) was evaluated as a candidate gene for a quantitative trait locus (QTL) on BTA29 affecting meat tenderness by characterization of nucleotide sequence variation in the gene. Single-nucleotide polymorphisms (SNP) were identified by sequencing all 22 exons and 19 of the 21 introns in two sires (Piedmontese × Angus located at the U.S. Meat Animal Research Center in Clay Center, NE; Jersey × Limousin located at AgResearch in New Zealand) of
more » ... dent resource populations previously shown to be segregating meat tenderness QTL on BTA29. The majority of the 38 SNP were found in introns or were synonymous substitutions in the coding regions, with two exceptions. Exons 14 and 9 contained SNP that were predicted to alter the protein sequence by the substitution of isoleucine for valine in Domain III of the protein, and alanine
doi:10.2527/2002.80123077x pmid:12542147 fatcat:osvmeyg4xzhubpcq44am4c77ee