Upregulated microRNA-16 as an oncogene in renal cell carcinoma

DUQUN CHEN, YIFAN LI, ZUHU YU, ZHENGMING SU, WENSHUI YU, YUCHI LI, SHANGQI YANG, YAOTING GUI, LIANGCHAO NI, YONGQING LAI
2012 Molecular Medicine Reports  
MicroRNAs (miRs) are small, endogenous noncoding RNAs that serve a significant function in various biologic processes, including those involved in cancer. The present study aimed to determine the expression and function of miR-16 in renal cell carcinoma (RCC). Quantitative polymerase chain reaction was used to quantify the expression of miR-16 in 48 paired RCC tissues and adjacent normal tissues. The impact of miR-16 on cell proliferation, migration and apoptosis was analyzed by transfecting
more » ... -16 mature molecules into the renal cancer cell lines 786-O and ACHN. The results indicated that miR-16 was significantly upregulated in RCC tissues (P<0.05). Downregulation of miR-16 resulted in reduced cell proliferation and migration and increased levels of apoptosis, while overexpression of miR-16 resulted in accelerated cellular proliferation and migration, suggesting that miR-16 may function as an oncogene in RCC. The present study demonstrated for the first time, to the best of our knowledge, that miR-16 is upregulated in RCC and acts as an oncogene by inducing cellular proliferation, migration and reducing apoptosis. Further study of miR-16 in RCC may clarify the molecular mechanisms of RCC carcinogenesis and aid in the development of novel biomarkers and therapeutic options.
doi:10.3892/mmr.2015.3496 pmid:25815587 fatcat:bbntzhd7xbb4dhzrjh3i5jzyia