Pseudomonas aeruginosa is the most rebellious hospital bug, causing life threatening diseasesoften challenging to treat. In view of this, a study was undertaken to uncover hemolytic P.aeruginosaand antibiotic susceptibility against selected antibiotics. Air samples from different locations within a hospital were collected by exposure plate technique and surface samples were collected by wiping the sterile moist swabs over the ventilator tube, bed railing, computer mouse/ keyboard,patient table,

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... floor and wall from intensive care unit. Collected samples were subjected to standard isolation, characterization and hemolytic assay procedures. ßhemolytic P.aeruginosa was isolated from the collected samples. Antimicrobial susceptibility test was performed accordingto CLSI (2015) against gentamicin (120µg), ampicillin (10µg), amoxicillin (10µg), imipenem(10µg), andceftazidime (30µg).P.aeruginosa was sensitive to all antibiotics tested. The most imperative preventive measure against contagion is to rev-up decontamination protocol and continuous education to generate better understanding about the reservoir of Pseudomonas areuginosa, which is routinely over looked.