The Alli (Nymphea nouchali Burm.f) is an aquatic herb, under the family Nymphaeaceae. As per Siddha classical literatures this plant possess astringent, refrigerant, antimicrobial actions. The aim of this study was to evaluate the phytochemical and antibacterial activities of the crude extract of rhizome and flowers of Alli (Nymphea nouchali Burm.f). Screening was performed for Carbohydrate, Alkaloids, Flavonoids, Glycosides, Proteins, Terpenoid, Tannin & Saponin. Antibacterial activity was

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... by agar disk diffusion test. The phytochemical screening showed positive results for Carbohydrate, Alkaloids, Flavonoids and tannins in the concentration of 68 0.44mg/100g, 51 0.88 mg/100g, 38 .49 mg/100g, and 41 0.23 mg/100g respectively. N.nouchali extract found to be effective with concentration of (1mg/ml) against S.aureus, E.coli, P.vulgaris, S.mutans & Klebsilla species suppressing their growth with inhibition zones of 14mm,13mm,11mm,10mm,8mm respectively. Alli (Nymphea nouchali Burm.f) demonstrated the presence of secondary metabolites which are known for their medicinal values.