Use of Raman spectroscopy to study reduction state of mitochondrial cytochromes in an isolated heart under normoxic and hypoxic conditions

N. A. Brazhe, M. Treiman, B. Faricelli, J. H. Vestergaard, O. V. Sosnovtseva
2013 European Heart Journal  
Mechanisms involved in ischaemia, metabolism and stress adaptation 607 hibitor 5-HD at a dose of 5 40 min before isc, 5) NaHS+ L-NAME treated with NaHS and the synthase of NO inhibitor L-NAME bolus at a dose of 10 on the 19th min of isc and 6) NaHS+ Wortmannin treated with NaHS and the PI3/AKT inhibitor wortmannin at a dose of 60μg kg -1 on the 19th min of isc. The infarct size and the area at risk were estimated (%I/R). In a second series of experiments, we determined the
more » ... tion of tissue AKT, ERK½, eNOS, GSK3β, STAT3 and phopsholamban (PLB) from Control, NaHS and NaHS+DT-2 groups. Results: NaHS reduced %I/R compared to Control (12.3±3.3% vs 46.4±1.8%, p<0.05), the addition of the DT2 abrogated this benefit (39.8±3.4%), 5-HD and L-NAME did not alter the infarct size reduction (14.1±2.0% and 14.7±2.2% respectively, p=NS vs NaHS) whilst wortmannin reversed it (41.8±1.4%, p=NS vs Control). Phosphorylation of AKT, ERK½, and PLB was higher in NaHS vs Control and NaHS+DT-2 groups with no eNOS and GSK3β activation. STAT-3 activation was higher in NaHS and NaHS+DT-2 vs Control. Conclusion: Exogenous administration of H2S induces pharmacological postconditioning via AKT/PKG/PLB and PKG/ ERK½ activation independently of eNOS, GSK3β, JAK/STAT and mitoKATP activation. P3271 | BENCH Pentraxin-3 and galectin-1 are key mediators of the cardioprotective paracrine effects exerted by fetal mesenchymal stem cells isolated from human placenta
doi:10.1093/eurheartj/eht309.p3274 fatcat:zqfdya7ewjejppvnysan4ocrqa