Porcine deltacoronavirus (PDCoV) infection antagonizes interferon-λ1 production

Shudan Liu, Puxian Fang, Wenting Ke, Jing Wang, Xunlei Wang, Shaobo Xiao, Liurong Fang
2020 Veterinary Microbiology  
Porcine deltacoronavirus (PDCoV) is a novel swine enteropathogenic coronavirus that causes watery diarrhea, vomiting and mortality in nursing piglets. Type III interferons (IFN-λs) are the major antiviral cytokines in intestinal epithelial cells, the target cells in vivo for PDCoV. In this study, we found that PDCoV infection remarkably inhibited Sendai virus-induced IFN-λ1 production by suppressing transcription factors IRF and NF-κB in IPI-2I cells, a line of porcine intestinal mucosal
more » ... ial cells. We also confirmed that PDCoV infection impeded the activation of IFN-λ1 promoter stimulated by RIG-I, MDA5 and MAVS, but not by TBK1 and IRF1. Although the expression levels of IRF1 and MAVS were not changed, PDCoV infection resulted in reduction of the number of peroxisomes, the platform for MAVS to activate IRF1, and subsequent type III IFN production. Taken together, our study demonstrates that PDCoV suppresses type III IFN responses to circumvent the host's antiviral immunity.
doi:10.1016/j.vetmic.2020.108785 pmid:32768229 pmcid:PMC7331541 fatcat:qk5kfc7x6beizekmezfg64be3q