The Relationship Of Lipids To Antithrombin Consumption During Clotting

J Winter, B Bennett, F McTaggart, A Douglas
1981 Thrombosis and Haemostasis  
The relationship between serum lipids and the plasma and serum anti thrombin activity was studied in blood samples from 18 patients who had suffered myocardial infarction 1-3 years previously and 18 age matched controls without clinical evidence of vascular disease. Progressive anti thrombin activity was measured by the technique of Abildgaard et al (1970) and anti thrombin III levels were measured by radial immunodiffusion using Partigen plates.Plasma specific anti thrombin activity was
more » ... as the ratio of progressive anti thrombin activity to the anti thrombin III level measured by immunoassay. Serum was separated from blood incubated for 1 hour at 37°C in glass tubes.Serum tryglycerides were determined using a commercial kinetic enzymatic method, heparin precipitable lipoproteins (VLDL and LDL) by nephephelometric method, and high density lipoprotein by a Mn2+ and heparin precipitation method. A negative correlation was found between plasma specific anti thrombin activity and serum triglycerides (R = -0.35 p < 0.05). Significant correlations were found between the consumption of anti thrombin activity during clotting and serum triglyceride (R = -0.53, p < 0.001) heparin precipitable lipoproteins (R = -0.48, p < 0.01) and high density lipoprotein (R = + 0.40, p < 0.01).These data indicate that blood lipids influence antithrombin III - coagulant interactions, possibly protecting the activated coagulant factors from inhibition by antithrombin. The data concerning anti thrombin-lipid interactions clearly merits expansion.
doi:10.1055/s-0038-1653118 fatcat:gl2356ntrbazrhv3xsodxpygti