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Branched Chain Amino Acid Transaminases in Rat Ascites Hepatoma Cells
29.ネズミ腹水腫瘍細胞の分岐鎖アミノ酸トランスアミナーセ
1968
Ikagaku Shinpojumu
29.ネズミ腹水腫瘍細胞の分岐鎖アミノ酸トランスアミナーセ
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A new branched chain amino acids (valine, leucine and isoleucine) -a-ketoglutaratetransaminase was isolated and characterized from rat ascites hepatoma cells (AH 130). The supernatant of the tumor homogenate was applied on DEAE cellulose column chromatography and two active fractions were eluted. The first fraction was eluted by 0.02 M phosphate buffer, and the enzymological properties (substrate specificity, Km values for the substrates, activation by
doi:10.14921/jscc1961.7.0_167
fatcat:2dpxlcfzcrcglgmsc2idxf62mm