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The emerging next-generation sequencing method based on PCR technology boosts genome sequencing speed considerably, the expense is also get decreased. It has been utilized to address a broad range of bioinformatics problems. Limited by reliable output sequence length of next-generation sequencing technologies, we are confined to study gene fragments with 30~50 bps in general and it is relatively shorter than traditional gene fragment length. Anchoring gene fragments in long reference sequencedoi:10.1186/1471-2105-10-s1-s17 pmid:19208116 pmcid:PMC2648759 fatcat:nbsdaallpfbknjgi5watc3eipi