Objective: To describe the process of construction and assessment of the content of a Virtual Learning Object on preventing bloodstream infections associated with infusion therapy. Method: Methodological study carried out in three stages: elaborating the technical, scientific content; assessing theoretical content; and constructing the Virtual Learning Object. Results: We produced the theoretical content of the Virtual Learning Object. Such content obtained an 85% agreement in assessment, which

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... was adequate. The final product comprised 39 content screens, which integrate the main recommendations and contain images, hypertexts, videos, case studies, and quiz, through an exclusive platform. Final Considerations: This study made it possible to portray the method for constructing and assessing the content of a Virtual Learning Object, enabling am easy application to health professionals, aiming at guiding them to quality care.