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Mutant of Escherichia coli K-12 Deficient for Detergent-Resistant Phospholipase A
1972
Journal of Bacteriology
enzyme is membrane-bound and the substrate is a lipid, a special procedure was developed for isolating mutants deficient for the enzyme from agar plates. A sodium dodecyl sulfate (SDS)sensitive mutant was used as a parental strain for the isolation of DR phospholipase A-deficient mutant. Soft agar containing an unsaturated fatty acid auxotroph and SDS was poured over colonies of the parental strain. The cells were easily solubilized with SDS, and phospholipids were efficiently digested by DR
doi:10.1128/jb.110.3.864-869.1972
fatcat:vehzx7dr4babdktiy3z4ppp4gu