What's in a Drop? Correlating Observations and Outcomes to Guide Macromolecular Crystallization Experiments

Joseph R. Luft, Jennifer R. Wolfley, Edward H. Snell
<span title="2011-03-02">2011</span> <i title="American Chemical Society (ACS)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/tfncqskjxjgbvilbdcq654of3m" style="color: black;">Crystal Growth &amp; Design</a> </i> &nbsp;
Observations of crystallization experiments are classified as specific outcomes and integrated through a phase diagram to visualize solubility and thereby direct subsequent experiments. Specific examples are taken from our highthroughput crystallization laboratory which provided a broad scope of data from 20 million crystallization experiments on 12 500 different biological macromolecules. The methods and rationale are broadly and generally applicable in any crystallization laboratory. Through
more &raquo; ... combination of incomplete factorial sampling of crystallization cocktails, standard outcome classifications, visualization of outcomes as they relate chemically, and application of a simple phase diagram approach, we demonstrate how to logically design subsequent crystallization experiments.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1021/cg1013945">doi:10.1021/cg1013945</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/21643490">pmid:21643490</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC3106348/">pmcid:PMC3106348</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/qxxvpzd575a5dlielz7isgdrmi">fatcat:qxxvpzd575a5dlielz7isgdrmi</a> </span>
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