The continuous low dose insulin and glucose infusion test: a simplified and accurate method for the evaluation of insulin sensitivity and insulin secretion in population studies

P. M. Piatti
1995 Journal of Clinical Endocrinology and Metabolism  
In this study we investigated a simple nonlabor-intensive method to evaluate insulin sensitivity and p-cell function which is suitable for application in population studies. The method is a refinement of the modified Harano test and consists of a continuous low dose insulin (25 mU/kgh) and glucose (4 mg/kg.min) infusion test (LDIGIT) lasting 150 min. Insulin sensitivity was evaluated as the MCR of glucose divided by the steady state serum insulin level achieved at the end of the test. Insulin
more » ... cretion was expressed as the incremental area for C-peptide concentration during the first 15 min of the test. We compared the indices of insulin sensitivity and insulin secretion yielded by LDIGIT with those derived from the euglycemic clamp and the hyperglycemic clamp, respectively. Fifty-four subjects underwent a LDIGIT (33 with normal glucose tolerance and 21 with imoaired glucose tolerance); ofthe 54;19 were submitted to a euglycemic clamp, 18 to a hyperglycemic clamp, and 10 to a modified Harano test (insulin infusion, 50 mU/kgh; glucose infusion, 6 mg/kg.min). LDIGIT over-
doi:10.1210/jc.80.1.34 pmid:7829636 fatcat:bhl4rjeudrdsxm4mrtixs3fifi