Three-Dimensional in Vivo Imaging of Green Fluorescent Protein-Expressing T Cells in Mice with Noncontact Fluorescence Molecular Tomography

Anikitos Garofalakis, Giannis Zacharakis, Heiko Meyer, Eleftherios N. Economou, Clio Mamalaki, Joseph Papamatheakis, Dimitris Kioussis, Vasilis Ntziachristos, Jorge Ripoll
2007 Molecular Imaging  
Given that optical tomography is capable of quantitatively imaging the distribution of several important chromophores and fluorophores in vivo, there has been a great deal of interest in developing optical imaging systems with increased numbers of measurements under optimal experimental conditions. In this article, we present a novel system that enables three-dimensional imaging of fluorescent probes in whole animals using a noncontact setup, in parallel with a three-dimensional surface
more » ... nal surface reconstruction algorithm. This approach is directed toward the in vivo imaging of fluorophore or fluorescent protein concentration in small animals. The system consists of a rotating sample holder and a lens-coupled charge-coupled device camera in combination with a fibercoupled laser scanning device. By measuring multiple projections, large data sets can be obtained, thus improving the accuracy of the inversion models used for quantitative three-dimensional reconstruction of fluorochrome distribution, as well as facilitating a higher spatial resolution. In this study, the system was applied to determining the distribution of green fluorescent protein (GFP)-
doi:10.2310/7290.2007.00007 pmid:17445504 fatcat:6qsr6dhpkrhjdow5yemxflyzyq