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Golden Gate Cloning-Compatible DNA Replicon/2A-Mediated Polycistronic Vectors for Plants
2020
Frontiers in Plant Science
The expression of multiple proteins and high-throughput vector assembly system are highly relevant in the field of plant genetic engineering and synthetic biology. Deployment of the self-cleaving 2A peptide that mediates polycistronic gene expression has been an effective strategy for multigene expression, as it minimizes issues in coordinated transgene regulation and trait staking in plants. However, efficient vector assembly systems optimized for 2A peptide-mediated polycistronic expression
doi:10.3389/fpls.2020.559365
pmid:33193484
pmcid:PMC7609577
fatcat:zvsqf7grcjd37l4gjzhvdzibtq