House dust mite allergens can cause allergic diseases, including asthma, atopic dermatitis and rhinitis. Der f 20 is a novel allergen of Dermatophagoides farina (Der f), which is an arginine kinase. In the present study, the B-cell and T-cell epitopes of Der f 20 were predicted. The protein attribution, patterns, physicochemical properties and secondary structure of Der f 20 were also predicted. Der f 20 is a member of the ATP:guanido phosphotransferase family and contains a phosphagen kinase

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... ttern. Using homology modeling, the present study constructed a reasonable tertiary structure of Der f 20. Using BcePred, ABCpred, BCPred and BPAP systems, B-cell epitopes at 20-25, 41-49, 111-118, 131-141, 170-174 and 312-321 were predicted. Using NetMHCIIpan-3.0 and NetMHCII-2.2, T-cell epitopes were predicted at 194-202, 239-247 and 274-282. These results provide a theoretical basis for the design off Der f 20 epitope-based vaccines. Abbreviations: HDM, house dust mite; PDB, Protein Data Bank; 3D structure, tertiary structure