The tuber and seed contain a high rich in protein. Direct multiple shoot bud induction was developed in Psophocarpus tetragonolobus used as cotyledon explants. MS media supplemented with various concentration of TDZ alone or along with IAA. MS media +TDZ (2.0 mg/L) minimum number of shoot buds were induced. MS media supplemented with TDZ (2.0 mg/L) in combination with IAA (0.4 mg/L) induced dark green compact callus with maximum number of shoot buds were obtained.