FIRST ENVIRONMENTAL DNA (EDNA) RECORD OF CENTRAL AMAZON IN A FLOODPLAIN LAKE: EXTRACTION METHOD SELECTION AND VALIDATION / PRIMEIRO REGISTRO DE DNA AMBIENTAL (EDNA) DA AMAZÔNIA CENTRAL EM UM LAGO DE VÁRZEA: SELEÇÃO E VALIDAÇÃO DE MÉTODO DE EXTRAÇÃO

Danniel Rocha Bevilaqua, Sabrina Araújo de Melo, Carlos Edwar de Carvalho Freitas, Ana Caroline Viana da Silva, Jacqueline da Silva Batista
2020 Brazilian Journal of Development  
In recent years, environmental DNA (eDNA) has emerged as an effective method for detecting aquatic organisms. The current study tested a method of DNA extraction from water samples from Central Amazonian floodplain lake. Water collections were performed in a floodplain lake on Paciência Island, on the Solimões River (Amazon Basin, Brazil) during the high water period. Seven commercial DNA extraction methods/kits were tested with samples collected from Lake Cacau. After quantification, eDNA
more » ... fication, eDNA samples were amplified with 12S and 16S
doi:10.34117/bjdv6n11-254 fatcat:pqhu7jgudrgizjlite3uyramzq