Currently, the concept of oro-dental prophylaxis and prevention is becoming more and more publicized among the population. These notions promote both the prevention of the development of dento-periodontal and oral structures diseases, diagnosing the diseases of the oral cavity and stopping their evolution, as well as the prevention of complications in the oro-maxillo-facial sphere. The purpose of this paper was to perform a comparative experimental study between the commensal oral flora and the

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... composition of the biofilm after tooth brushing with Sensodyne Rapid Relief toothpaste and Lacalut toothpaste with hydroxyapatite. The study group included a number of 15 patients, of which 9 men and 6 women, aged between 20 and 71 years. The collection of biological preparations took place in the dental office, where the patients presented themselves for an individualized treatment plan, and the analysis and interpretation of the results were carried out in the Microbiology Discipline, "Carol Davila" University of Medicine and Pharmcy, Bucharest, Romania. According to the results of our study, following dental brushing with Lacalut toothpaste with hydroxyapatite, its effectiveness was demonstrated on species such as G+ lanceolate cocci, curved G+ bacilli, G- spindle bacilli or G- filamentous formations, while the effect antibacterial for G+ shells in diplo and short chains was not as expected. Sensodyne Rapid Relief Toothpaste has been shown to have a positive effect on species of G+ lanceolate cocci, G- fusiform bacilli, round-headed G+ bacilli, curved G+ bacilli or G- filamentous formations. On the other hand, on G+ cocci in diplo and short chains and epithelial cells it did not show the desired efficiency, these being found in the highest percentage. This study once again shows the importance of microscopic examination in assessing the effect of some toothpastes on the weight of the oral flora.