Effects of anesthetics pentobarbital sodium and chloral hydrate on urine proteome

Mindi Zhao, Xundou Li, Menglin Li, Youhe Gao
2015 PeerJ  
Urine can be a better source than blood for biomarker discovery since it accumulates many changes. The urine proteome is susceptible to many factors, including anesthesia. Pentobarbital sodium and chloral hydrate are commonly used anesthetics in animal experiments. This study demonstrated the effects of these two anesthetics on the rat urine proteome using liquid chromatography-tandem mass spectrometry (LC-MS/MS). With anesthesia, the urinary protein-to-creatinine ratio of all rats increased
more » ... fold. The relative abundance of 22 and 23 urinary proteins were changed with pentobarbital sodium or chloral hydrate anesthesia, respectively, as determined by label-free quantification. Among these changed proteins, fifteen had been considered as candidate biomarkers such as uromodulin, and sixteen had been considered stable in healthy human urine, which are more likely to be considered as potential biomarkers when changed, such as transferrin. The pattern of changed urinary proteins provides clues to the discovery of urinary proteins regulatory mechanisms. When determining a candidate biomarker, anesthetic-related effects can be excluded from future biomarker discovery studies. Since anesthetics take effects via nervous system, this study is the first to provide clues that the protein handling function of the kidney may possibly be regulated by the nervous system. Subjects Biochemistry Supplemental information for this article can be found online at http://dx.doi.org/ 10.7717/peerj.813#supplemental-information. REFERENCES Abe M, Tokunaga T, Yamada K, Furukawa T. 1988. Gamma-aminobutyric acid and taurine antagonize the central effects of angiotensin II and renin on the intake of water and salt, and on blood pressure in rats. Neuropharmacology 27:309-318 .x. Curtis P, Evens S, Resnick J, Thompson CJ, Rimer R, Hisley J. 1989. Patterns of uterine contractions and prolonged uterine activity using three methods of breast stimulation for contraction stress tests. Obstetrics and Gynecology 73:631-638. Cutillas PR, Chalkley RJ, Hansen KC, Cramer R, Norden AG, Waterfield MD, Burlingame AL, Unwin RJ. 2004. The urinary proteome in Fanconi syndrome implies specificity in the reabsorption of proteins by renal proximal tubule cells. Dare TO, Davies HA, Turton JA, Lomas L, Williams TC, York MJ. 2002. Application of surface-enhanced laser desorption/ionization technology to the detection and identification of urinary parvalbumin-alpha: a biomarker of compound-induced skeletal muscle toxicity in the rat. Electrophoresis 23:3241-3251
doi:10.7717/peerj.813 pmid:25789206 pmcid:PMC4362303 fatcat:kiqdlpuxmjhtvdtylohfkkjjaa