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Background SARS-CoV-2 Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) colorimetric detection is a sensitive and specific point-of-care molecular biology technique used to detect the virus in only 30 min. In this manuscript we have described a few nuances of the technique still not properly described in the literature: the presence of three colors clusters; the correlation of the viral load with the color change; and the importance of using an internal control to avoiddoi:10.1186/s10020-021-00289-0 pmid:33771097 pmcid:PMC7996115 fatcat:w3zmrvllivd3ndcamjx7n5ppsm