Experimental studies simulating ambithermic condition during renal ex vivo surgery were performed by means of histopathology, electron microscopy, microangiography, tissue culture, LDH in perfusate and renal autotransplantation in dogs. As for perfusates at the time of initial flushing, lactate Ringer solution, hyperosmolar intracellular electrolyte solution (Sacks solution) and mannitol added hyperosmolar (430 mOsm) lactate Ringer solution were selected. These experiments demonstrated relative

more »

... superiority of Sacks solution over the other solution in preventing renal deterioration during renal ambithermia. Although renal ischemic damage during ambithermic condition was prevented by flushing ice-cold Sacks solution after nephrectomy, renal protective effect of Sacks solution was not complete if ischemic time exceeds 6 hours and suggested the necessity of cumbersome surface cooling during surgery in such cases.