Behavior study of substances is often performed by different methods to provide a lot of information about these substances. The present study describes behavior of cefixime trihydrate (CEF-3H2O) which was carried out by pH buffer solutions and different polarity solvents. Solutions with different pH were showed different UV absorption spectra for each pH, revealed a red shifted as compared with UV absorption spectra of drug in less pH (2) as reference. Different polarity solvents of water,

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... anol and ethanol were used and appeared different UV absorption spectra that showed a bathochromic shift behavior considering UV absorption spectra of water as reference, all spectra intersects at certain  289 nm (isosbestic point). Hypsochromic shift (blue shift) of cefixime trihydrate occur with solvents of HCl and NaOH and showed isosbestic point at 254 nm.