Nuclear male sterility (NMS) is a very important character for cotton breeding and genetics programs. Identification of linked molecular markers with NMS will greatly facilitate breeding for this trait. Among all of the Nuclear male-sterile genes, only Dong-A (ms 14 ) was utilized successfully in hybrid production of cotton. Dong-A (ms 14 ), a recessive NMS line developed from spontaneous mutation in upland cotton, has been applied most widely and played an increasing role in hybrid cultivar

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... elopment in China. An interspecific F 2 population comprised of 180 individual plants was developed by crossing a cultivar of Kang A (Dong-A derived Lines, Gossypium hirsutum L.) to a cultivar of 601588 (G. barbadense L.). Basing on this population the ms 14 gene was mapped on chromosome 2 and closely linkaged with BNL3971 marker within a genetic distance of 16.7 cM. The BNL3971 marker could be used for the marker assisted selection in breeding a new cultivar line, and provide the information for closely location and further gene isolation by map based cloning.