The effect of extracellular matrix on myogenic induction of adipose mesenchymal stem cells
Bankground: As one of the hot cells in the field of regenerative medicine, adipose mesenchymal stem cells(ADSCs) have been proved to have the ability of myoblast differentiation, but the disadvantage is that the efficiency of myoblast differentiation is not very high.Extracellular matrix(ECM), as a mixture of cytokines and proteins secreted by cells, is the substance secreted by cells. It has the advantages of non-toxic and harmless, and can provide the necessary material and environmental
... environmental basis for cell growth and development.This study was to explore whether the myogenic differentiation potential of ADSCs cultured in ECM was improved.Method: ADSCs were extracted from subcutaneous fat of male SD rats at the age of 3 months and weight 250g. The third generation cellular of ADSCs were prepared for ECM,and the ECM was contained in the culture bottle for amplify the ADSCs. Myogenic induction was performed by 5-azacytidine using the same generation of ADSCs cultured with ECM and non-ECM.Result: ECM can significantly increase the rate of cell proliferation (P<0.05).Immunofluorescence detected the expression of β-actin, Myod and Desmin in cells ,and showed no significant difference in the expression of β-actin between groups (P>0.05). However, there were significant differences in Myod and Desmin between groups (P<0.001).RT-PCR showed that the mRNA expressions of myosin heavy chain(MHC), troponin, Myogein and Myf5 were significantly different among groups (P<0.001).Conclusion: ECM culture of ADSCs can improve its growth rate and myogenic differentiation potential.