Effect of growth environment on Pseudomonas aeruginosa killing by rabbit polymorphonuclear leudocytes and cationic proteins

J E Finch, M R Brown
1978 Infection and Immunity  
Pseudomonas aeruginosa grown in a chemostat under carbon-and magnesiumlimited conditions showed varying resistance to killing by rabbit peritoneal exudate polymorphonuclear leukocytes. Slow-growing (D = 0.05 h-'), magnesiumlimited cells were significantly more resistant to the lethal effects of the phagocytes than were fast-growing magnesium-limited cells and carbon-limited cells (D = 0.05 h-1 and D = 0.5 h-', respectively). The resistance of magnesium-limited cells to killing by cationic
more » ... ns isolated from the leukocytes was shown to be growth-rate dependent, the slowest-growing (D = 0.05 h-') cells being the most resistant. Carbon-limited cells were sensitive to killing by the cationic proteins at all growth rates tested. Antisera raised in rabbits to all types of cells and commercial anti-Pseudomonas serum rapidly agglutinated magnesium-limited cells but failed to agglutinate carbon-limited cells. There was some indication that slow-growing (D = 0.05 h-'), magnesium-limited cells agglutinated most readily with both types of antisera. No difference was detected in the mouse toxicity of heat-killed cells grown under the various conditions.
doi:10.1128/iai.20.2.340-346.1978 fatcat:bdvdew7tgvh3bamefvaoshdx5q