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Thrombopoietin-Induced Stimulation of Megakaryocyte- Enriched Bone Marrow Cultures
VIIth International Congress on Thrombosis and Haemostasis
Liquid cultures of bone marrow cells enriched for megakaryocytes were assayed for incorporation of 3H-thymidine (3H-TdR) into acid-precipitable cell digests to determine the effect of thrombopoietin on DNA synthesis. As previously described, thrombopoietin was prepared by ammonium sulfate fractionation of pooled plasma obtained from thrombocytopenic rabbits. A control fraction was prepared from normal rabbit plasma. The thrombopoietic activity of these fractions was determined in vivo withdoi:10.1055/s-0039-1687198 fatcat:owgv5v7kcngqpnlyeqbjsdwfgu