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Developing a Cell Culture System from Nile Tilapia (Oreochromis Niloticus, L.) Ovarian Tissue in Egypt
2012
IOSR Journal of Agriculture and Veterinary Science
Cell culture from ovarian tissue of healthy female fingerlings Oreochromis niloticus was established with average weight of 20 to 30 gm/female. Three culture media were screened and tested: Leibowitz L-15), Minimum essential medium (MEM) and RPMI 1640 medium. Cells were maintained at 16, 27 or 30 o C for screening the favorable optimally incubation temperature. Culture medium was refreshed every 5 days. Among the three tested media, L-15 supplemented with 20% (FBS) supported higher cells
doi:10.9790/2380-0120812
fatcat:ivuie3d7izcwnpkqluud5bkwd4