Developing a Cell Culture System from Nile Tilapia (Oreochromis Niloticus, L.) Ovarian Tissue in Egypt

Omaima Khamiss
2012 IOSR Journal of Agriculture and Veterinary Science  
Cell culture from ovarian tissue of healthy female fingerlings Oreochromis niloticus was established with average weight of 20 to 30 gm/female. Three culture media were screened and tested: Leibowitz L-15), Minimum essential medium (MEM) and RPMI 1640 medium. Cells were maintained at 16, 27 or 30 o C for screening the favorable optimally incubation temperature. Culture medium was refreshed every 5 days. Among the three tested media, L-15 supplemented with 20% (FBS) supported higher cells
more » ... higher cells attachment leading to cell proliferation than MEM and RPMI that didn't enhance the cell development and no cell proliferation was obtained with them. At different incubation temperature no proliferation were obtained when cells were incubated at 16 o C compared with 27 o C and 30 o C. This work is one of the rare reports of a cell culture system establishment in Egypt and unique from the ovarian tissue of O. niloticus. By using this technique, it should now be possible to develop different method of curing treatments and pathogens diagnosis because the fish can be bred in captivity and fingerlings reared under laboratory conditions. Developing a cell culture system from Nile tilapia (Oreochromis niloticus, L.) ovarian tissue in Egypt 9 | P a g e
doi:10.9790/2380-0120812 fatcat:ivuie3d7izcwnpkqluud5bkwd4