The expression of PGC-1αin the mice brain after traumatic brain injury

You-Wu Fan, Kang-Jian Sun, Lei Mao, Hong Liao, Han-Dong Wang
2012 Brain Injury  
Peroxisome proliferator activated receptor co-activator-1 (PGC-1) is a transcriptional co-activator that co-ordinately regulates genes required for mitochondrial biogenesis and is a key contributor to the up-regulation of antioxidant activities in response to oxidative stress. The expression pattern of PGC-1 after traumatic brain injury (TBI) has not been studied. Materials and methods: Ninety male ICR mice (28-32 g) were randomly assigned to six groups: sham, 3, 6, 12, 24 and 48 hours after
more » ... d 48 hours after TBI. PGC-1 mRNA levels in mice brain were detected by reverse-transcriptase polymerase chain reaction and its nuclear protein levels by Western blot from 3-48 hours after TBI. PGC-1 distribution in the cerebral cortex after TBI was investigated by immunohistochemistry. Main outcomes and results: The PGC-1 mRNA level significantly increased from 3 hours after TBI, peaked at 6 hours and gradually decreased from 12 to 48 hours. The nuclear PGC-1 protein level increased from 6 to 24 hours after TBI and decreased at 48 hours after TBI. Increased PGC-1 immunostaining was detected in the neurons of the cerebral cortex at 12 hours after TBI. Conclusion: PGC-1 may play an important role in the brain after TBI.
doi:10.3109/02699052.2012.672789 pmid:22642418 fatcat:eivhlqzjefbrljv2csg6e4eiqm