Large amounts of microorganisms are emitted from animal houses into the environment via exhaust air. To assess the potential risks, the spread of microorganisms can be simulated with computer models. Such modeling usually does not take into account die-off rates, since there are hardly any reliable data so far on how long microorganisms can survive in outdoor air. Previous studies were conducted almost exclusively in closed chambers and usually only took into account the influence of individual

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... environmental factors such as temperature or humidity. Therefore, a novel bioaerosol chamber was developed to quantify the survival rates of Staphylococci specific to livestock under outdoor air conditions. For evaluation, the survival rates of Staphylococcus xylosus were determined as a function of temperature, relative humidity, ozone concentration, and global radiation. Survival rates decreased with increasing temperature, decreasing relative humidity, increasing global radiation intensity, and increasing ozone concentration. At 12 min in the airborne state, die-off rates of more than 90% were observed, especially at high global radiation levels > 400 W/m2. The novel bioaerosol chamber enabled the investigation of the survival rates of airborne microorganisms over a certain period of time in a quasi-closed system and yet under real outdoor air conditions.