2P089 Directed evolution of membrane protein, alpha hemolysin, by development of liposome display method(01F. Protein: Engineering,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))
2P089 膜たんぱく質の進化工学手法、リポソームディスプレイ法によるα-ヘモリシンのin vitro分子進化(01F. 蛋白質:蛋白質工学/進化工学,ポスター,第52回日本生物物理学会年会(2014年度))

Satoshi Fujii, Tomoaki Matsuura, Takeshi Sunami, Yasuaki Kazuta, Tetsuya Yomo
2014 Seibutsu Butsuri  
Biomolecules such as protein, peptide and nucleic acids interact with each other in the cell controlling its life activities. Therefore, techniques to reproduce biomolecular interactions correctly on the solid substrate would be a good versatile way to clarify the biological phenomena. In this research, we investigated the influence of protein orientation and density on peptide-protein interaction (adopting cysteine synthase (CS) complex formation as the model) detection with employing site
more » ... ific protein immobilization technique. As the results, consideration of the location of functional site with avoiding the steric hindrance by adjusting the protein density and the degree of freedom of the peptide would be important to design higher interaction detection system. 2P086 無機基板表面を標的としたラクダ抗体から着想するスマート なバイオセンサー仕様抗体の設計
doi:10.2142/biophys.54.s209_5 fatcat:txh6inzmxjdpndzu55wh35ahyy