PCR-based DNA Profiling of Human Y Chromosomes [chapter]

Fabrício R. Santos, Denise R. Carvalho-Silva, Sérgio D. J. Pena
1999 DNA Profiling and DNA Fingerprinting  
Until recently, the use of human Y chromosome DNA polymorphisms for evolutionary studies and in human identification had been cramped by a scarcity of suitable markers. Fortunately this picture has changed after the discovery of an extensive range of new polymorphisms in the past five years. These are convenient markers, easily amplified by PCR, that fall into two large groups: slow evolving biallelic markers (single nucleotide polymorphisms and retroelement insertion polymorphisms) and fast
more » ... phisms) and fast evolving multiallelic tandem repeat polymorphisms (microsatellites and minisatellites). In this review we provide simplified detection protocols for the typing of the most important PCR-based polymorphisms and discuss their use in population genetic studies and forensic casework, emphasizing the need to adequate the rate of evolution and information content of the marker to the specific application intended. 3
doi:10.1007/978-3-0348-7582-0_9 fatcat:jgcmcv3tszfwjkpy3a3seoecua