An Overview on (–)-Hydroxycitric Acid in Obesity Regulation [chapter]

Shirley Zafra-Stone, Gary Grover, Harry Preuss, Debasis Bagchi, Manashi Bagchi
2007 Obesity  
Hydroxycitric acid (HCA), a major active ingredient of Garcinia cambogia extracts, is known to suppress body weight gain and fat synthesis in animals and humans. But the underlying mechanism of HCA action is not fully understood. Clinical study on 100 obese individuals for a period of 3 months was performed followed by a computational study aimed to investigate the effects of HCA treatment on human subjects at anthropometric and plasma lipid profile levels. A detailed hepatic metabolic model
more » ... metabolic model was used to incorporate the effect of HCA at the metabolic pathway level. Perturbation analysis of ATP citrate lyase activity in the metabolic pathway was performed to simulate the net effect of HCA. Significant reductions in body weight, triceps, subscapular, and mid axillary measurements as well as in serum triglyceride, cholesterol, HDL and LDL levels were observed following HCA dosage. During the study, half of the subjects experienced a decline in body weight and the remainder experienced an increase in body weight. However, analysis of fat mass with the help of empirical correlations clearly showed significant reduction in the mean values due to HCA dosage in both cases. An extra increase in fat free mass was responsible for offsetting the decrease in fat mass for the subjects who experienced an increase in body weight during the trials. Perturbation analysis showed a net reduction in fatty acid, triglyceride and cholesterol synthesis along with urea cycle fluxes under lipogenetic conditions. Moreover, protein synthesis fluxes increased under these conditions. These results indicate that HCA treatment can reduce body weight gain and fat accumulation in obese subjects along with improving their anthropometric parameters and metabolic state. Fig. 8 Relative flux map for HCA effect under lipogenetic conditions (with glucose, FA, AA ¼ 2 folds each) vs. normal fasting conditions (with glucose, FA, AA ¼ 1 fold each) at time ¼ 66 minutes. All fluxes are relative except where two rates are mentioned for the same reaction, these numbers are absolute and indicate change in reaction direction due to HCA effect (values mentioned in red). Rest of the fluxes are for the case when citrate to OAA + ACoA are reduced by 44% & FA oxidation fluxes are decreased by 43% at the end of 66 minutes relative to when there is no such perturbation. 18586 | RSC Adv., 2019, 9, 18578-18588 This journal is
doi:10.1201/9781420005479.ch27 fatcat:tyqxgphksnebzdk4l7xjfecpq4