Advances in yeast genome engineering

Florian David, Verena Siewers
2014 FEMS Yeast Research  
Genome engineering based on homologous recombination has been applied to yeast for many years. However, the growing importance of yeast as a cell factory in metabolic engineering and chassis in synthetic biology demands methods for fast and efficient introduction of multiple targeted changes such as gene knockouts and introduction of multistep metabolic pathways. In this review, we summarize recent improvements of existing genome engineering methods, the development of novel techniques, for
more » ... ple for advanced genome redesign and evolution, and the importance of endonucleases as genome engineering tools.
doi:10.1111/1567-1364.12200 pmid:25154295 fatcat:lmc4texumrcl7g6nmfjpyjsd6a