HSV-1-derived amplicon vectors: recent technological improvements and remaining difficulties - a review

Alberto Luis Epstein
2009 Memórias do Instituto Oswaldo Cruz  
Amplicons are defective and non-integrative vectors derived from herpes simplex virus type 1. As the vector genome carries no virus genes, amplicons are both non-toxic for the infected cells and non-pathogenic for the inoculated organisms. In addition, the large transgenic capacity of amplicons, which allow delivery of up to 150 Kbp of foreign DNA, makes these vectors one of the most powerful, interesting and versatile gene delivery platforms. We present here recent technological developments
more » ... ical developments that have significantly improved and extended the use of amplicons, both in cultured cells and in living organisms. In addition, this review also discusses the many difficulties still pending to be solved, in order to achieve stable and physiologically regulated transgene expression.
doi:10.1590/s0074-02762009000300002 pmid:19547864 fatcat:tbcmduolbngxblyihhnua23yeu