Pathogen-Reactive T Helper Cell Analysis in the Pig

Friederike Ebner, Patrycja Schwiertz, Svenja Steinfelder, Robert Pieper, Jürgen Zentek, Nicole Schütze, Christoph G. Baums, Gottfried Alber, Peter Geldhof, Susanne Hartmann
<span title="2017-05-17">2017</span> <i title="Frontiers Media SA"> <a target="_blank" rel="noopener" href="" style="color: black;">Frontiers in Immunology</a> </i> &nbsp;
There is growing interest in studying host-pathogen interactions in human-relevant large animal models such as the pig. Despite the progress in developing immunological reagents for porcine T cell research, there is an urgent need to directly assess pathogenspecific T cells-an extremely rare population of cells, but of upmost importance in orchestrating the host immune response to a given pathogen. Here, we established that the activation marker CD154 (CD40L), known from human and mouse
more &raquo; ... identifies also porcine antigen-reactive CD4 + T lymphocytes. CD154 expression was upregulated early after antigen encounter and CD4 + CD154 + antigen-reactive T cells coexpressed cytokines. Antigen-induced expansion and autologous restimulation enabled a time-and dose-resolved analysis of CD154 regulation and a significantly increased resolution in phenotypic profiling of antigen-responsive cells. CD154 expression identified T cells responding to staphylococcal Enterotoxin B superantigen stimulation as well as T cells responding to the fungus Candida albicans and T cells specific for a highly prevalent intestinal parasite, the nematode Ascaris suum during acute and trickle infection. Antigen-reactive T cells were further detected after immunization of pigs with a single recombinant bacterial antigen of Streptococcus suis only. Thus, our study offers new ways to study antigen-specific T lymphocytes in the pig and their contribution to hostpathogen interactions.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="">doi:10.3389/fimmu.2017.00565</a> <a target="_blank" rel="external noopener" href="">pmid:28567041</a> <a target="_blank" rel="external noopener" href="">pmcid:PMC5434156</a> <a target="_blank" rel="external noopener" href="">fatcat:xgkf7ndvgrfjnidan2yeiwy4qy</a> </span>
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