Objective: This study aimed to determinelevels of expression ET-1 in serum and renal tissue associated with changes in kidney structure. Method: The rats were divided into two groups. The Obese group was given a high-fat-induced diet chow and a standard diet chow for the control group. ET-1 protein expression and localization in the glomerulus of the kidney were examined. The serum of blood checked for ET-Iusing ELISA method and assessed the ET-1 expression of the glomeruliby

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... y (IHC). Mesangial expansion is an indicator to evaluate the degree of glomerular damage by Periodic Acid-Schiff(PAS staining). Associations between the immunoreactivity for ET-1 in glomeruli and the degree of kidney injury were examined. Results: Lee Index was significantly higher in the obese group than in the control group (p<0.05). ET-1 immunoreactivity was considerably higher in the obese group than the control rat. It was a strong correlation with enhancingmesangial expansion glomeruli using PAS staining of the renal tissue (rs=0.765; p= 0.001). Contrast to ET-1 levels in the serum revealed no significant in obese rat groups (p>0.05). Conclusion: These data confirm the role of ET-1 will provide changes in the levels of glomeruli impairment, but serum levels may well fail to reflect the local expression at the tissue level.