Identification of Wine Yeasts by PCR-RFLP without Previous Isolation on Plate

Juan Carlos Espinosa, Monica Fernandez-Gonzalez, Juan Ubeda, Ana Briones
2002 Food Technology and Biotechnology  
The population of wine yeasts during spontaneous must fermentation was characterized by direct 5.8S-ITS rDNA region amplification without previous plate isolation or enrichment. RFLP analysis was applied to each of the amplification products detected, and the corresponding yeast identifications were made. The method provides a fast and direct way of determining yeast population present during wine fermentation.
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