Distinct and stage-specific contributions of TET1 and TET2 to stepwise cytosine oxidation in the transition from naive to primed pluripotency [article]

Christopher B Mulholland, Franziska R Traube, Edris Parsa, Eva-Maria Eckl, Maximillian Schoenung, Miha Modic, Michael D Bartoschek, Paul Stolz, Joel Ryan, Thomas Carell, Heinrich Leonhardt, Sebastian Bultmann
2018 bioRxiv   pre-print
The TET-oxidized cytosine derivatives, 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), are considered DNA demethylation intermediates as well as stable epigenetic marks in mammals. We compared modified cytosine and enzyme levels in TET-knockout cells during naive pluripotency exit and found distinct and differentiation-dependent contributions of TET1 and TET2 to 5hmC and 5fC formation. The divergent modified cytosine levels argue for independent consecutive oxidation steps in vivo
more » ... on steps in vivo with broad implications for epigenetic regulation.
doi:10.1101/281519 fatcat:gqh37yzo3zd7bdgeg2g7l2npm4