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Small-Conductance Calcium-Activated K+ Channels Are Expressed in Pancreatic Islets and Regulate Glucose Responses
2003
Diabetes
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RESEARCH DESIGN AND METHODS Isolation and culturing of mouse islets of Langerhans and islet cells. Islets were isolated from the pancreas of 1-to 5-month-old C57BL/J6 mice (Jackson Laboratories) using collagenase digestion and Ficoll gradients as previously described (12) . Islets were cultured in RPMI-1640 medium supplemented with 10% FCS, 11.6 mmol/l glucose, 100 IU/ml penicillin, and 100 g/ml streptomycin (islet complete medium [ICM]) for 2-5 days. To obtain dissociated islet From the
doi:10.2337/diabetes.52.8.2000
pmid:12882916
fatcat:og7o3fxixbgxbehegp5f3igeqi