Small-Conductance Calcium-Activated K+ Channels Are Expressed in Pancreatic Islets and Regulate Glucose Responses

N. A. Tamarina, Y. Wang, L. Mariotto, A. Kuznetsov, C. Bond, J. Adelman, L. H. Philipson
2003 Diabetes  
RESEARCH DESIGN AND METHODS Isolation and culturing of mouse islets of Langerhans and islet cells. Islets were isolated from the pancreas of 1-to 5-month-old C57BL/J6 mice (Jackson Laboratories) using collagenase digestion and Ficoll gradients as previously described (12) . Islets were cultured in RPMI-1640 medium supplemented with 10% FCS, 11.6 mmol/l glucose, 100 IU/ml penicillin, and 100 g/ml streptomycin (islet complete medium [ICM]) for 2-5 days. To obtain dissociated islet From the
doi:10.2337/diabetes.52.8.2000 pmid:12882916 fatcat:og7o3fxixbgxbehegp5f3igeqi