Role of the Cytosolic Phospholipase A2-linked Cascade in Signaling by an Oncogenic, Constitutively Active Ha-Ras Isoform

Min-Hyuk Yoo, Chang-Hoon Woo, Hye-Jin You, Sung-Hoon Cho, Byung-Chul Kim, Ji-Eun Choi, Jang-Soo Chun, Byung H. Jhun, Tae-Sung Kim, Jae-Hong Kim
2001 Journal of Biological Chemistry  
Activation of Ras signaling by growth factors has been associated with gene regulation and cell proliferation. Here we characterize the contributory role of cytosolic phospholipase A 2 in the oncogenic Ha-Ras V12 signaling pathway leading to activation of c-fos serum response element (SRE) and transformation in Rat-2 fibroblasts. Using a c-fos SRE-luciferase reporter gene, we showed that the transactivation of SRE by Ha-Ras V12 is mainly via a Rac-linked cascade, although the
more » ... Raf-mitogen-activated protein kinase cascade is required for full activation. In addition, Ha-Ras V12 -induced DNA synthesis was significantly attenuated by microinjection of recombinant Rac N17 , a dominant negative mutant of Rac1.
doi:10.1074/jbc.m101975200 pmid:11323430 fatcat:pfuywxphwrfybo4bwydy6cge5y