Larvae, pupae and adults of Gymnetis rufilateris (Illiger, 1800) (Coleoptera: Cetoniidae: Cetoniinae) were collected in the municipality of Rochedo, Mato Grosso do Sul State. Studies on biology and morphology were conducted at the Laboratory of Entomology of the Universidade Estadual de Mato Grosso do Sul in Aquidauana, Mato Grosso do Sul, Brazil. Females of couples formed in the laboratory oviposited and allowed the biology studies. The eggs lasted for 15.5 days. Larvae of the 1st, 2nd and 3rd

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... instars lasted 13.5, 30.6 and 113.1 days, respectively. The pupal period lasted 12.8 days. The egg-to-adult period lasts 186.8 days. The larvae fed on decomposing feces of poultry. In the laboratory, adults fed on ripe banana (Musa sp.) (Musaceae). Descriptions of the 3rd larval instar and pupa of G. rufilateris are presented. A key to known larvae of Gymnetis is also included.